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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
Human HIF-1 alpha ELISA Kit
Human HIF-1 alpha ELISA Kit from Abcam
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Citations:
(11)
Supplier Page
Supplier Page from
Abcam for
Human HIF-1 alpha ELISA Kit
Product Specs
Company Info
Citations
Reviews
Bench Tips
Product Specs
Item
Human HIF-1 alpha ELISA Kit
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab171577
Quantity
1 96TESTS
Reactivity
Human
Applications
ELISA
NCBI Gene Aliases
HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Detection Target
HIF-1 alpha
Sensitivity
42 pg/ml
Detection Range
0.23 - 15 ng/ml
Type
Sandwich (quantitative)
Gene Name
HIF1A
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Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (11)
(1)
Carvacrol Arrests the Proliferation of Hypopharyngeal Carcinoma Cells by Suppressing Ornithine Decarboxylase and Hyaluronidase Activities
Frontiers in Nutrition
April 8, 2022
Kaneez Fatima, Suaib Luqman, Abha Meena
by a kit purchased from ELab Sciences, USA. The VEGF (Catalog No.
ab100663
) and HIF-1α (Catalog No.
ab171577
) were estimated using a kit procured from
Abcam
, UK. Cell Cycle Analysis, Dichloro-Dihydro-Fluorescein
(2)
Inhibition of HMGB1 Might Enhance the Protective Effect of Taxifolin in Cardiomyocytes via PI3K/AKT Signaling Pathway
Iran J Pharm Res
September 27, 2021
Erjun Feng, Jian Wang, Xinwei Wang, Zhenguo Wang, Xiaochu Chen, Xu Zhu, Wenli Hou
ab75762
) were purchased from
Abcam
.
FGF21
(
ab222506
), VEGF-α (
ab119566
), TGF-β (
ab100647
), HIF-1α (
ab171577
) ELISA kits were purchased from
Abcam
(Cambridge, UK). Construct of vectors. Full-length of HMGB1 cDNA
(3)
Optimal Hypoxic Preconditioning of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells (hES-MSCs) and Their Characteristics
International Journal of Stem Cells
May 30, 2021
Seung Min Lee, Dae Won Jun, Hyeon Tae Kang, Ju Hee Oh, Waqar Khalid Saeed, Sang Bong Ahn
under normal and low O2 conditions were collected in a microtube, and 1× Cell Extraction Buffer PTR (
ab171577
,
Abcam
, Cambridge, UK) was used for
protein extraction
. The protein extracts were quantified, and 20
(4)
Low oxygen tension differentially regulates the expression of placental solute carriers and ABC transporters
FEBS Letters
March 1, 2021
Ludwik Gorczyca, Jianyao Du, Kristin M. Bircsak, Xia Wen, Anna M. Vetrano, Lauren M. Aleksunes
analysis. HIF-1α protein concentration was determined by HIF-1α ELISA kit, as per manufacturer’s protocol (
ab171577
,
Abcam
). Data were normalized to protein concentration.
Western Blot
. Following treatment, BeWo cells
(5)
Hypoxia Regulates DPP4 Expression, Proteolytic Inactivation, and Shedding from Ovarian Cancer Cells
International Journal of Molecular Sciences
October 30, 2020
Laura R. Moffitt, Maree Bilandzic, Amy L. Wilson, Yiqian Chen, Mark D. Gorrell, Martin K. Oehler, Magdalena Plebanski, Andrew N. Stephens
cell culture
supernatant were performed using commercially available ELISA kits (Human HIF-1-alpha;
ab171577
and
Human
CD26;
ab11951
,
Abcam
, Cambridge, UK) as per the manufacturer’s instructions. CD26 ELISA was
(6)
Expression and predictive value of HIF‑1α and VEGF in patients with burns following treatment
Experimental and Therapeutic Medicine
October 5, 2020
Zhufeng Liu, Ling Liu, Xuejuan Cheng, Liming Gao
for 10 min to separate the serum and then stored in the refrigerator at -80˚C. ELISA kits (cat. nos.
ab171577
and
ab233625
;
Abcam
), was employed to determine HIF-1α and VEGF levels. The dilution concentrations
(7)
Association of Nuclear Receptor Coactivators with Hypoxia-Inducible Factor-1
α
in the Serum of Patients with Chronic Kidney Disease
BioMed Research International
August 20, 2020
Tianbiao Zhou, Wenshan Lin, Shujun Lin, Zhiqing Zhong, Yuanyuan Luo, Zhijun Lin, Weiji Xie, Weitao Shen, Kai Hong
enzyme-linked immunosorbent assay
method, and all the reagents were purchased from
Abcam
, Co., USA (HIF-1α:
ab171577
; VEFG:
ab222510
) and Dogesce (NCOA1: DG95699Q; NCOA2: DG95703Q; and NCOA3: BioMed Research International DG95709Q).
(8)
Chemically Induced Hypoxia Enhances miRNA Functions in Breast Cancer
Cancers
July 22, 2020
Gervin E, Shin B, Opperman R, Cullen M, Feser R, Maiti S, Majumder M.
Enzyme-Linked Immunosorbent Assay
(
ELISA
) Analysis of HIF-1α HIF-1α protein quantification was carried out using the
ab171577
-HIF1a Human SimpleStep ELISA Kit (
Abcam
, Toronto, ON, Canada). This
assay
is specific to the HIF-1α
(9)
The brains of six African mole-rat species show divergent responses to hypoxia
Journal of Experimental Biology
January 1, 2020
Samantha M. Logan, Kama E. Szereszewski, Nigel C. Bennett, Daniel W. Hart, Barry van Jaarsveld, Matthew E. Pamenter, Kenneth B. Storey
ELISA
HIF-1α protein abundance was determined with a commercially available HIF-1α ELISA kit (
Abcam
,
ab171577
). All buffers, reagents and standards were prepared according to the manufacturer’s instructions. First,
(10)
Longer Hydroxyurea Administration Prior to Imatinib Mesylate is Risk Factor for Unsuccessful Major Molecular Response in Chronic-Phase Chronic Myeloid Leukemia: Possibility of P-Glycoprotein Role
Asian Pacific Journal of Cancer Prevention
December 1, 2019
Ikhwan Rinaldi, Ary Harryanto Reksodiputro, Sri Widia Jusman, Alida Harahap, Rianto Setiabudy, Septelia Inawati Wanandi, Karmel Tambunan, Catharina Suharti
evaluated by enzymed-linked immunosorbent
assay
(
ELISA
) using a kit from
Abcam
with a catalogue number of
ab171577
. The examination of P-gp expression was carried out using quantitative real-time reverse transcription
(11)
Ascorbic acid attenuates cell stress by activating the fibroblast growth factor 21/fibroblast growth factor receptor 2/adiponectin pathway in HepG2 cells
Molecular medicine reports
September 1, 2019
Gu X, Luo X, Wang Y, He Z, Li X, Wu K, Zhang Y, Yang Y, Ji J, Luo X.
of cells. The concentration of HIFα was measured using a commercially available ELISA kit (cat. no.
ab171577
,
Abcam
) according to the manufacturer's instructions.
RNA interference
. The small interfering RNA (siRNA) sequences
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Reviews
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
Human HIF-1 alpha ELISA Kit from Abcam
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